MCQs: Animal Cell Culture.

MCQs: Animal Cell Culture.

Quiz

  

Time for this test is 45 minutes.

 

1. Who is called the father of tissue culture?
   1.   Harrison
   2.   Arnold
   3.   Ross
   4.   Roux
2. Optimum pH required for the growth of mammalian cells is,
   1.   5.3-7.0
   2.   6.5-7.0
   3.   7.2-7.4
   4.   8.1-8.9
3. The optimum temperature of any cell or organ is called,
   1.   Critical temperature
   2.   Threshold temperature
   3.   Ceiling temperature
   4.   None of the above
4. Which of the following is the oldest cell line,
   1.   Hela cell line
   2.   Vero cell line
   3.   CHO cell line
   4.   BHK cell line
5. Credit of first successful animal cloning goes to,
   1.   Arnold
   2.   Ross
   3.   Roux
   4.   Wilmut
6. Blood serum is plasma without…..
   1.   Fibrin
   2.   Fibrinogen
   3.   Collagen
   4.   Oxygen
7. First successfully cloned animal is a,
   1.   Lion
   2.   Tiger
   3.   Goat
   4.   Sheep
8. Artificial serum containing media contains ------% serum in it.
   1.   0-10
   2.   5-20
   3.   20-50
   4.   30-50
9. Serum is one of the good source of nutrients still has many disadvantages, identify one from following,
   1.   Expensive
   2.   Varies batch to batch.
   3.   Source of contamination.
   4.   All of the above.
10. Artificial medias are prepared to overcome disadvantages of Serum containing medias, still has many disadvantages, identify one from following,
    1.   Slower growth.
    2.   More preparation time.
    3.   Specific for one cell type
    4.   All of the above.
11. Osmolarity between --------is good for many cell lines.
    1.   160 m Osm/kg and 220 m Osm/kg
    2.   260 m Osm/kg and 320 m Osm/kg
    3.   360 m Osm/kg and 420 m Osm/kg
    4.   460 m Osm/kg and 520 m Osm/kg
12. In the beginning the cell suspension is isolated and inoculated in a new culture vessel with fresh culture medium, such culture is called,
    1.   Primary Cell Culture
    2.   Secondary Cell Culture
    3.   Cell line
    4.   None of the above.
13. Which of the following stages is not performed while isolating a primary culture,
    1.   Isolation of the tissue
    2.   Cell counting.
    3.   Disaggregation of the tissue
    4.   Seeding of culture into the culture vessel
14. Identify the inexpensive, rapid and simple method used for disaggregation.
    1.   Physical disaggregation.
    2.   Enzymatic disaggregation.
    3.   Using chelating agents
    4.   None of the above.
15. Identify the method for disaggregation which offers high recovery of cells without affecting the viability of cells.
    1.   Physical disaggregation
    2.   Enzymatic disaggregation
    3.   Using chelating agents
    4.   None of the above
16. The process of enzymatic disaggregation of tissues using trypsin is called….
    1.   Trypsinization
    2.   Chymotrypsinzation
    3.   Isotrypsinization
    4.   Helotrypsinization
17. Which of the following methods of enzymatic disaggregation has an improved survival rate for the cells.
    1.   Cold Trypsinization.
    2.   Warm trypsinization
18. Which of the following types of cells are cultivated in liquid medium?
    1.   Anchorage dependent cells.
    2.   Anchorage independent cells.
19. Identify from the following the example of Anchorage Dependent Cells”.
    1.   Kidney
    2.   Liver
    3.   Pancreas
    4.   All of the above.
20. Identify from the following the example of Anchorage Independent Cells”.
    1.   Kidney
    2.   Liver
    3.   Pancreas
    4.   Skeletal muscle cells.