Simple Pharmanotes

  Introduction: Definition : Sterilization is defined as complete removal of microorganisms from an object, surface or a product. Chemical Sterilization Causes of removal of microorganisms by the use of chemical bactericidal agents. Although the physical methods of sterilization are more appropriate it is not possible to use them in certain conditions e.g. in case of thermolabile materials , in such cases Chemical sterilization is preferred. However compatibility between chemical agent and substance to be sterilized must be first taken into the consideration. It is important to follow safety rules in the workplace safety during the use of chemical agents. The chemical method of sterilization can be classified as,  liquid sterilization. gaseous sterilization Gaseous Sterilization: Ethylene oxide: Ethylene oxide (EO) gas is a common gas used for chemical sterilization because of its wide range of compatibility . This method is a widespread method used for almost 70% of all sterilization

  Introduction: Definition : Sterilization is defined as complete removal of microorganisms from an object, surface or a product. Sterilization by filtration is the preferred method of sterilizing thermolabile liquid and gases.  It simply removes microbes instead of killing them.  It is the method of choice for sterilizing antibiotic solutions , toxic chemicals , radioisotopes , vaccines , and carbohydrates, which are all heat-sensitive . Mechanism of Filtration Sterilization: Filters work by physically trapping particles larger than the pore size and by retaining somewhat smaller particles via electrostatic attraction of the particles to the filters.  Besides porosity, other factors also influence the efficiency of filtration, they are: electric charge of the filter electric charge carried by the organisms nature of the fluid being filtered Biological indicator:  Micromonospora  diminuta. Procedure: The solutions to be sterilized is passed through the filter and collected in the ste